Basic Principles of Chromatography

There are four main types of chromatography These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography Liquid Chromatography is used in the world to test water samples to look for pollution in lakes and rivers It is used to analyze metal ions and organic compounds in solutions Liquid

Chromatography The Classification of Chromatography

alumina is an example of adsorption chromatography (Fig ) The solvent that is put into a column is called the eluent, and the liquid that flows out of the end of the column is called the eluate Adsorption chromatography using a column Partition In partition chromatography the stationary phase is a non-volatile liquid which is held as a thin

Basics of chromatographic Techniques Course 1

adsorption chromatography) is also considered to be a normal phase separation method Because of its versatility and wide range of applicability, reversed-phased chromatography is the most frequently used hplc method Types Partition Chromatography

CHAPTER 1 2 3 Introduction, Chromatography Theory, and

Liquid chromatography can further be 13 divided into ion exchange, separations based on size, and even extended to gel-14 based electrophoretic techniques This book will provide a basic introduction to 15 different types of liquid and gas chromatography The relationship between each 16 type of chromatography is illustrated in Figure 1 1 17

Principles of Chromatography

Liquid Chromatography Column separation (liquid-liquid, liquid-solid) used for separating and analyzing compounds based on differences in their interaction with a stationary phase Adsorption, partition, ion exchange, molecular exclusion and affinity

Module 5 Chromatographic Techniques Lecture 28: Principles of

2 Pump: One or two pump to flow the buffer from reservoir Different types of pumps are used in chromatography system, mostly based on the pressure level required to perform chromatography A pump is chosen as per the pressure required to run the mobile phase Based on the pressure level, liquid chromatography can be

Separation techniques: Chromatography

chromatography, gel permeation chromatography, high-pressure liquid chromatography, and affinity chromatography [6] Types of chromatography • Column chromatography • Ion-exchange chromatography • Gel-permeation (molecular sieve) chromatography • Affinity chromatography • Paper chromatography • Thin-layer chromatography