Replication of the virus occurs within the cytoplasm of infected cells and is not known to undergo genetic recombination or integration into the cellular genome.
Similar to Ad-based vector, recombinant VSV is able to accommodate relatively large inserts and multiple genes in its genome..
Biosafety Level 2 (BSL-2) for activities with materials and cultures known or reasonably expected to contain VSV. experimentally infected animals, an N95 respirator is required for procedures done outside a BSC or other containment device.Jun 20, 2023.
CAUTION: Vesicular stomatitis virus Indiana strain is a Biosafety Level 2 (BSL-2) pathogen.
Follow all appropriate guidelines and regulations for the use and handling of pathogenic microorganisms.
We recommend that all procedures with virus be performed in a certified biological safety cabinet (BSC)..
Vesicular stomatitis virus (VSV) is a nonsegmented, negative-strand RNA virus and the prototype of the Rhabdoviridae family.
VSV encodes 5 structural proteins: nucleocapsid protein (N), phosphoprotein (P), matrix protein (M), the surface glycoprotein (G), and the RNA-dependent RNA polymerase (L) (19)..
VSVs are classified in the order Mononegavirales (non-segmented-negative-sense RNA viruses), family Rhabdoviridae, genus Vesiculovirus..
Recombinant vesicular stomatitis virus (VSV) vectors are widely used for a variety of applications including studying mechanisms of viral entry into host cells, identification of cellular receptors utilized by viruses for cell entry, screening of viral entry inhibitors and vaccine development research..
VSV has been shown to replicate preferentially in tumor cells (36).
This is due to the fact that many cancer cells have defects in host antiviral response pathways, mainly the interferon pathway, which makes these cells unable to effectively combat and suppress viral replication..
Indiana, Cocal, Alagoas, New Jersey, Isfahan and Maraba viruses are classified as Risk Group 2 human pathogens.
Vesicular Stomatitis Virus is classified as Risk Group 3 since the species includes Risk Group 3 serotypes.Jan 12, 2012.
The microbes in a BSL-4 lab are dangerous and exotic, posing a high risk of aerosol-transmitted infections.
Infections caused by these microbes are frequently fatal and without treatment or vaccines.
Two examples of microbes worked with in a BSL-4 laboratory include Ebola and Marburg viruses..
Replication of the virus occurs within the cytoplasm of infected cells and is not known to undergo genetic recombination or integration into the cellular genome.
Similar to Ad-based vector, recombinant VSV is able to accommodate relatively large inserts and multiple genes in its genome..
Vesicular Stomatitis Virus and Related Vesiculoviruses
They can infect a large variety of cells and are therefore tremendously useful for gene transduction.
VSV is also used as an expression vector in candidate vaccines..
Biosafety features related to the use of vectors derived from Herpesviruses, particularly HSV-1 were detailed in the current review of Lim et al. [ 27 ].
Several strategies have been developed mediating tumor-specificity and avoiding the dissemination of the RCVV vectors in non-tumor cells.
If a person does become infected, they typically develop mild flu-like symptoms or remain asymptomatic; therefore VSV is considered fairly safe for use in humans ( Suder et al., 2018 ).
Finally, VSV is easily propagated in a wide variety of mammalian cell lines on a large-scale basis.
Risks include direct contact with skin and mucous membranes of the eye, nose and mouth, parenteral inoculation, ingestion.
1) High energy-creating activities (centrifugation, sonication, high pressure systems, vortexing, tube cap popping).
2) Handling of sharps (needles, scalpels, microtome blades, broken glass, etc.).
3) Splash/droplet-creating acti.
Third generation and higher lentiviral vectors used in labs are frequently pseudotyped with envelope glycoprotein from vesicular stomatitis virus VSV-G.
In this case, BSL2 containment is implemented since these viruses have the capability of transducing human cells.
There are still conditions when BSL2-enhanced precautions are used:.
While, the default biological safety containment level for recombinant viruses is Biosafety Level (BSL- 2) and Animal Biosafety Level 2 (ABSL-2), there are exceptions.
A lower biosafety containment level may suffice for incomplete viruses cultured in vitro. • A few animal and human viruses qualify for lower biosafety containment.
