Sop biosafety cabinet

  • What is SOP biosafety?

    These guidelines outline the safe usage of Biohazardous materials and the equipment associated with them to ensure adequate control of contamination..

  • What is the EN standard for biosafety cabinets?

    The primary standards for biosafety cabinets are NSF/ANSI 49 and EN 12469, with NSF/ANSI 49 being the "gold standard" in the US, and EN 12469 being used in Europe.
    These standards provide cabinet construction and performance guidelines to ensure a safe working environment..

  • What is the use of biosafety cabinet sop?

    Place supplies, equipment and papers well back from the front of the cabinet, positioned so that air intake or exhaust grills are not obstructed.
    Avoid bringing non-essential equipment and supplies into the cabinet.
    Work towards the middle of the cabinet, at the air split or at least 6 inches from the front grill..

  • What procedures can be conducted in a biosafety cabinet?

    Use of Biological Safety Cabinets (BSC)
    Conduct all procedures involving the manipulation of potentially infectious materials, including aerosol-producing activities, when using aerosol-producing equipment, or when using high concentrations/large volumes of organisms, inside a biological safety cabinet..

  • The daily maintenance of the Bio Safety Cabinet (BSC) should be performed by lab staff and include:

    1Surface decontamination of the work zone.
    2) Cabinet power-up alarm verification.
    3) Removal of unnecessary items from the BSC.
    4) Flow check (inflow and downflow velocity)5Smoke pattern test.
    6) Entry in the logbook (shown in Table 2)
  • Disinfect cabinet surfaces with an appropriate disinfectant.
    Periodically remove the work surface and disinfect the area beneath it (including the catch pan) and wipe the surface of the UV light with disinfectant.
    Turn off the blower and fluorescent lamp.
    Do not turn on the UV lamp.
Avoid bringing non-essential equipment and supplies into the cabinet. Work towards the middle of the cabinet, at the air split or at least 6 inches from the front grill. Discard contaminated waste including contaminated gloves INSIDE the cabinet. Do not allow contaminated gloves outside of the cabinet.
Place only the necessary materials onto the cabinet work surface. Page 2. SOP #1127.5. Biological Safety Cabinets. Effective 12/16. Page 
This procedure outlines the use and maintenance of a laminar flow BSC used to obtain the optimum control over product quality while reducing the potential 
Wear gloves for hand protection. Surface decontaminate all items required and place into the cabinet. Set up the workflow from clean to dirty with the work area in the middle (see Figure 1). Keep bulky items such as biohazard bags and pipette trays and boxes towards the back and side of the cabinet.

Are open-flames necessary in a biological safety cabinet?

The Centers for Disease Control and Prevention (CDC) reports that “open-flames are not required in the near microbe-free environment of a biological safety cabinet” and create “turbulence which disrupts the pattern of air supplied to the work surface," jeopardizing the sterility of the work area.

What is a biological safety cabinet (BSC) SOP?

SOP # EHS-BIOS-201 version 1.0 This SOP applies to all McGill University personnel operating a Biological Safety Cabinet (BSC) as primary containment of risk group 2 and above and infectious materials.
This document shall be made accessible in each individual user laboratory.

What is a Class I Biological Safety Cabinet?

Class I Biological Safety Cabinet (Note:

  • Class I BSCs are manufactured on a limited basis.
    Most have been replaced with Class II BSCs.) The Class I BSC provides personnel and environmental protection, but no product protection.

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