Protein crystallography ppt

The first and least certain step in crystallography of a protein is obtaining crystals of the protein of interest

Obtaining suitable amounts of the protein of interest is usually carried out in a straightforward manner using established molecular biology techniques such as molecular cloning and affinity chromatography

Moreover, the requirement for pure, homogeneous, and stable protein solutions in crystallizations makes X-ray crystallography beneficial in other fields of protein research as well

Here, we describe the technique of X-ray protein crystallography and the steps involved for a successful three-dimensional crystal structure determination