X-ray crystallography practice problems

How do you prepare a sample for X ray crystallography?

.

1. Grind the sample to a talc-like powder (\x26lt;0
.062 mm).
2. Place a small amount of sample in the center of a quartz or single crystal quartz disk
3. Add 2–3 drops of acetone or distilled water to the sample
4. Spread the sample to a thin layer with a glass rod (rolling it over the sample works well)

How do you prepare a sample for X-ray crystallography?

.

1. Grind the sample to a talc-like powder (\x26lt;0
.062 mm).
2. Place a small amount of sample in the center of a quartz or single crystal quartz disk
3. Add 2–3 drops of acetone or distilled water to the sample
4. Spread the sample to a thin layer with a glass rod (rolling it over the sample works well)

How do you solve phase problems in X ray crystallography?

For small molecules, and a few proteins that form particularly well-ordered crystals, Patterson and direct methods provide a mathematical solution to this problem.
For larger biological molecules, MIR and MAD provide an effective experimental approach to phasing provided appropriate crystals can be grown..

How do you solve phase problems in X-ray crystallography?

For small molecules, and a few proteins that form particularly well-ordered crystals, Patterson and direct methods provide a mathematical solution to this problem.
For larger biological molecules, MIR and MAD provide an effective experimental approach to phasing provided appropriate crystals can be grown..

What are the problems in X ray crystallography?

Disadvantages of X-ray crystallography include: The sample must be crystallizable.
The types of sample that can be analyzed are limited.
In particular, membrane proteins and large molecules are difficult to crystallize, due to their large molecular weight and relatively poor solubility..