Serial crystallography method

  • What are the steps in protein crystallography?

    Protein Crystallization Steps

    Choose an appropriate solvent. Achieve supersaturation. Crystallize the product by controlling CPPs. Allow the system to reach equilibrium. Filter and dry the purified product..

  • Introducing the sample to precipitating agent can promote the nucleation of protein crystals in the solution, which can result in large three-dimensional crystals growing from the solution.
    There are two main techniques to obtain crystals: vapor diffusion and batch crystallization.
Serial crystallography is a method for obtaining structural information on an atomic level of a protein, without the need for large protein crystals. Instead, small diffraction datasets are collected on many small protein crystals, which are usually easier to obtain than large ones.

How does serial crystallography work?

The serial crystallography method comprises many steps (Darmanin et al

, 2016 )

The sample is delivered via fixed target holders or as a continuous stream of liquid to the region of interaction with the X-ray beam (Schlichting, 2015; Berntsen et al

, 2019 )

What is a robust statistical method for serial crystallography data analysis?

The algorithm is based on robust statistical methods

We have described a framework with application to serial crystallography data analysis, which is a particularly data intensive field

This algorithm uses robust statistical methods to reduce the number of input parameters and avoid the need for a priori knowledge of the experiment

What methods are used to find peaks in serial crystallography?

Most of the current methods used to perform peak finding and data reduction in serial crystallography are heuristic methods

An example is reported by Li & Zatsepin (2018 ), who uses a simple global threshold to separate the background signal from the Bragg peaks


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