Acceptable specimens for the Pap Test include cervical, cervical and vaginal, endocervical, vaginal and/or vulvar scrapings or brushings.
Specimens may be submitted as conventional smears, although the preferred method is the ThinPrep\xae Pap test..
In cytological smear, the sample is either smeared on a glass slide or made on a monolaye, and it is subsequently stained with the following stains- Papanicolaou, Diff quick and heamatoxylin and eosin.
Samples of CB are fixed in 10% neutral buffered formalin and then treated as a tissue block..
The method of Pap smear collection. (a) Long end of the Szalay's plastic spatula is seen fixed firmly in the endocervical canal and rotated clockwise through 360\xb0, (b) The long end collects cells from the squamocolumnar junction even if it is higher up in the canal.Mar 29, 2022.
In a Pap test, a vaginal speculum holds the vaginal walls apart to show the cervix.
Next, a sample of cells from the cervix is collected using a small cone-shaped brush and a tiny plastic spatula (1 and 2).
The brush and spatula are rinsed in a liquid-filled vial (3) and the vial goes to a laboratory for testing..
Of the four main types of squamous cervical (superficial, intermediate, parabasal and basal), superficial, and intermediate cells represent the overwhelming majority in a conventional smear.
Parabasal cells occur less frequently, and basal cells are exceedingly rare.
Endocervical cells sometimes are seen..
There are now three recommended options for cervical cancer screening in individuals aged 30–65 years: primary hrHPV testing every 5 years, cervical cytology alone every 3 years, or co-testing with a combination of cytology and hrHPV testing every 5 years Table.
1) All three screening strategies are effective, and each provides a reasonable balance .
Guidelines for Pap smear testing frequency vary between the United States Preventative Service Task Force (USPSTF) and American College of Obstetricians and Gynecologists (ACOG) but is recommended for women between the ages of 21 and 65.
Screening is not a recommendation under the age of 21.
A pap smear result will tell if the cells from the cervix are normal or abnormal.
When the test shows a normal result, patients may have nothing to worry about..
They should also inform the doctor know if they are pregnant or have had the test performed before and had abnormal results or are taking any kind of medication like birth control ..
Pap smear:
