Cytology with reflex

  • Is HPV reflex better than co testing?

    Co-testing with cytology and HPV testing (regardless of cytology result) detects more disease than either test alone; approximately twice as many cases of cervical dysplasia and cancers as cytology alone(3) and up to 16 percent more cases than primary HPV testing(4).May 1, 2022.

  • What does HPV DNA probe with reflex to genotype mean?

    A positive high-risk HPV test result indicates that the patient may be infected with one or more of the following HPV genotypes: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68, which are associated with cervical cancer and its precursor lesions; however, cross-reactions with other genotypes may occur..

  • What does reflex high risk HPV mean?

    A positive high-risk HPV test result indicates that the patient may be infected with one or more of the following HPV genotypes: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68, which are associated with cervical cancer and its precursor lesions; however, cross-reactions with other genotypes may occur..

  • What does reflex mean in Pap smear?

    Reflex testing—If you had an HPV test, a Pap test may be done on the same cells used for the HPV test.
    If you had a Pap test, an HPV test may be done on the same cells used for the Pap test.
    This is called reflex testing..

  • A positive high-risk HPV test result indicates that the patient may be infected with one or more of the following HPV genotypes: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68, which are associated with cervical cancer and its precursor lesions; however, cross-reactions with other genotypes may occur.
Reflex cytology on self‐samples for detecting abnormal cytology showed a sensitivity of 26.4% (95% CI 21.8–31.3) and specificity of 90.5% (95% CI 87.7–92.8). Of all ≥CIN2 cases, 29.4% (95% CI 22.5–37.1) were detected with reflex cytology on self‐samples.
The clinical utility of using reflex cytology on hrHPV‐positive self‐sampled material as triage for detecting ≥ASC‐US or HSIL is shown in Table 2. Of all hrHPV  AbstractIntroductionResultsDiscussion

Can reflex cytology be used as a triage test?

However, the Bethesda nomenclature and the CIN histologic grading system are internationally accepted terminologies.
Although reflex cytology on self‐samples could be used as an additional triage test, it cannot replace additional cytology testing at the general practitioner after a hrHPV ‐ positive self‐sample.

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Can reflex cytology detect CIN2?

Of all ≥CIN2 cases, 29.4% (95% CI 22.5–37.1) were detected with reflex cytology on self‐samples.
The positive predictive value for detection of ≥CIN2 was higher with cytology on self‐collected samples than on physician‐collected samples.
Of women who were lost to follow up, 12.9% were found to have abnormal cytology on their self‐sampled material.

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Is reflex cytology of added value for direct referral of women for Colposcopy?

Tweetable abstract:

  1. Reflex cytology for triage of hrHPV + self-samples is of added value for direct referral of women for colposcopy

Keywords:Cervical cancer; cervical intraepithelial neoplasia; human papillomavirus; screening; self-sampling; triage.
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What is the sensitivity of reflex cytology on self-samples?

Results:

  1. Reflex cytology on self-samples for detecting abnormal cytology showed a sensitivity of 26
4% (95% CI 21.8-31.3) and specificity of 90.5% (95% CI 87.7-92.8).
Of all ≥CIN2 cases, 29.4% (95% CI 22.5-37.1) were detected with reflex cytology on self-samples.

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