In this, a sample is vortexed and strained to break the mucus and large cell groups and then is treated through a density gradient centrifugation process to remove blood and debris.
The cell pellet is resuspended and is allowed to sediment onto a glass slide.
This is followed by staining on the PrepStain instrument..
Cytology is the exam of a single cell type, as often found in fluid specimens.
It's mainly used to diagnose or screen for cancer.
It's also used to screen for fetal abnormalities, for pap smears, to diagnose infectious organisms, and in other screening and diagnostic areas..
Liquid Based Cytology (LBC) is a new technique for collecting cytological samples in order to detect cervical cancer.
With conventional cytology a smear taker takes a sample that is applied directly to a slide for microscopic investigation..
General features of LBP compared with conventional / direct smears (Diagn Cytopathol 2007;35:621, Diagn Cytopathol 2013;41:257): Fragmentation of cell groups (clusters, papillary structures) Smaller cells and nuclei.
More discohesive cells.Mar 3, 2022.
However, liquid-based cytology rinses cervical cells in preservatives so that blood and other potentially obscuring material can be separated.
It also allows for additional testing of the sample, such as for human papillomavirus (HPV)..
Cytology is the exam of a single cell type, as often found in fluid specimens.
It's mainly used to diagnose or screen for cancer.
It's also used to screen for fetal abnormalities, for pap smears, to diagnose infectious organisms, and in other screening and diagnostic areas..
Background: Liquid-based cervical cytology was developed to improve the diagnostic reliability of Papanicolaou (Pap) smears.
Conventional Pap smears can have false-negative and false-positive results because of inadequate sampling and slide preparation, and errors in laboratory detection and interpretation..
Cellularity and Adequacy:
ThinPrep preparation is a filter-based cell concentration technique.
Two types are available, TP 2000 and a newer version TP 5000 for use only with TP.
Both are FDA-approved for processing Pap test and non-gyn specimens.
The TP 2000 processor is a semiautomated device which processes one specimen at a time.
TP 5000 is a fully automated benchtop ins.
In LBP, unlike the conventional smears, the acquired patient sample, from various anatomic sites, is rinsed in specially designated collection preservative media ensuring collection of potentially 100 % of the cell sample.
Liquid-based preparations (LBP) are increasingly being used for both gynecologic and non-gynecologic (non-gyn) cytology including:
Currently, only SP and TP have FDA approval since they are the more commonly used LBP.
The TP and SP preparations were approved for cervicovaginal (Pap test) cytology in 1996 and 1999, respectively, and both have since also been used for non-gynecological cytology including:
