Cytology feces

  • How do you do a fecal cytology?

    Smear a small quantity of faeces on a clean microscope slide.
    Mix with a few drops of water or physiological saline.
    Place a coverslip over the smear.
    The faecal material should not be left in a lump in the centre of the coverslip but evenly spread so that the microscope illumination can shine through..

  • What is fecal smear?

    Fecal smear is a laboratory test of a stool sample.
    This test is done to check for bacteria and parasites..

  • What is stool cytology?

    Normal cytology
    Fecal smears are of very low cellularity.
    No nucleated cells should be seen.
    A mixed population of bacteria should be present.
    Additionally, digesta can be observed.
    In a rectal scrape, in addition to the bacteria, clusters of rectal epithelium should be observed..

  • The key to a good fecal smear is to start with as fresh a sample as possible.
    Since Giardia is identified by observing the organism swimming across the slide, a direct fecal smear should be examined within 30 minutes of collection, before the organisms die or disappear.
Fecal smears are very easy to obtain and perform; only a small amount of fecal material is necessary to get a useful sample. A swab can be taken from fecal material or a gentle swab of the rectum. The swab is then rolled on a glass slide. Once the material is dried, the slide can be stained.
Summary. Dry-mount fecal cytology obtained by digital rectal examination or rectal scrape or recto-colonic lavage may help in the diagnosis of gastrointestinal diseases such as infectious, parasitic, inflammatory, or neoplastic conditions.

How do you store feces for a cytology test?

For most fecal tests, appropriate storage methods are available.
Cytology preparations may be fixed in ethanol or stained with preservative stains.
Refrigeration adequately stabilizes feces up to 24 hours for flotation or sedimentation procedures.
Formalin fixation can preserve feces for longer periods for flotation and/or sedimentation.

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How should fecal cytology be interpreted?

Since some fecal pathogens can be morphologically indistinguishable from incidental nonpathogens, results of dry-mount fecal cytology should be interpreted in the context of the patient’s clinical presentation and results of other diagnostic tests, such as:

  1. wet-mount fecal cytology
  2. bacterial culture
  3. fecal antigen detection methods
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What does wet mount fecal cytology reveal?

Wet mount fecal cytology may reveal characteristic curved organisms with “swarm of bees” like motility.
Dry mount fecal cytology often reveals increased fecal leukocytes (Fig 3).
Campylobacter may be identified on dark field or phase contrast microscopy.


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