Grapevines Carrying GFLV Coat Protein and Antifreeze Genes Institute of Genetic Engineering 2232 Kostinbrod-2, Bulgaria Received: December 20, 2000 ACKNOWLEDGEMENTS We thank Dr C Kontz and Dr R Golles for kindly providing the anti-freeze constructs and CP GFLV construct, respectively Georgy P GUTORANOV Ivan J TSVETKOV Violeta M COLOVA
Rootstock Development • 37 did not allow reproduction (egg mass formation evaluated with staining) and were advanced to testing against: – Two strains of RKN selected from Harmony rootstock – HarmA (M arenaria), HarmC (M incognita), and Xiphinema index (the dagger nematode vector of GFLV)
virus (GFLV) strain F13 (F13-cp) and the neomycin phosphotransferase (nptII) gene, one of the most widely used selectable marker genes in plant genetic engineering (Jelenic, 2003; Turrini et al , 2015)
GRAPE ROOTSTOCK TRANSFORMATION 227 Genesa Constructsb 1) LR3 43K double 35S AIMV GLRaV3-43K NOS 2) LR3cpST NPI 2enh 35S AIMV GLRaV3-CP 35S 3) LR3cpAS NPTII 2enh 35S AIMVI GLRaV3-CP 135S 4) FLcpST enh 35S AIMV GFLV-CP 35S 5) FLcpAS NPTII 2enh I35S AIMVI GFLV-CP 35S 6) FLcp+GUS NPTII 355S CMV GFLV-CP 35S 7) virE2 delB NPTII double 35S AIMV virE2
where genetic engineering is one of the few means available to solve a major agronomic problem or to study funda-mental mechanisms of plant biology, as a first step For instance, Grapevine fanleaf virus (GFLV), which is transmitted by soil nematodes, is the cause of an often lethal disease of grapevines with worldwide distribution
is the GFLV sense translatable coat protein gene in pGA482G 5) FLcpAS is the antisense form of the GFLV coat protein gene in pGA482G 6) FLcp + GUS is the GFLV sense translatable coat protein gene in pGA482GG 7) ritE2 del B is from Agrobacterium tumefiL- ciens in pBinl9 ~'From left to right: NPTII,
NR Rootstock CP gene GLRaV-2; GLRaV-3; GFLV Virus resistance 05/2005 05/2009 Niagara; Concord Drr206 Pisum sativum Fungal resistant 06/2004 06/2014 Chardonnay Thompson Seedless; Rootstock Saint George Polygalacturonase inhibitor protein P communis Fungal resistant 11/2007 09/2008 V vinifera Fungal resistant
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virE2 AGROBACTERIUM TUMEFACIENS - Springer
Genetic engineering offers an attractive possibility for generating disease-resistant plants; however, transformation of grapevine has been difficult Recently, transformation of grape was reported (Bari- bauh et al , 1990; Mullins et al , 1990; Le Gall et al , 1994; Nakano et Cited by : 66
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Breeding Rootstocks for California Vineyards
Breeding and Genetic Strategies • Evaluate and characterize germplasm • Crosses to develop new varieties and for genetic analysis • Evaluation of selections in greenhouse and field • Genetic mapping and genomics efforts to allow marker-aided selection, and localization and characterization of genes • Perhaps eventual genetic engineering with grape genes Rootstock Development
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California Grape Rootstock Research Foundation American
California Grape Rootstock Improvement Commission California Grape Rootstock Research Foundation American Vineyard Foundation CDFA Improvement Advisory Board California Table Grape Commission January 2014 Project Title: Development of next generation rootstocks for California vineyards Principal Investigator: Andrew Walker, Dept Viticulture and Enology, UC Davis 530-752
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California Grape Rootstock Improvement Commission
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In Vitro Cell Dev Biol-Plant 35:226-231, May-June 1999
Plant materials The rootstock cultivars Couderc 3309 (3309C) (V riparia X V rupestris), Vitis riparia 'Gloire de Montpellier' (Gloire), Teleki 5C (5C) (V berlandieri X V riparia), Millardet et De Grasset 101-14 (101-14 MGT) (V riparia X V rupestris), and Richter 110 (ll0R) (V rupestris X V berlan-dieri) were used in our experiments Initial
in old grape-growing areas as the vector of Grapevine fanleaf virus (GFLV), the first rotundifolia with a biotechnologically engineered resistance, such as the the rootstocks Freedom, 101-14 and Teleki 5C with this gene has been reported
. F