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DNA ligase is an important cellular enzyme, as its function is to repair broken phosphodiester bonds that may occur at random or as a consequence of DNA
? Ligases join nucleic acid molecules together ? Polymerases make copies of molecules ? Modifying enzymes remove or add chemical groups
NPTEL – Bio Technology – Genetic Engineering Applications DNA ligase enzyme is used by cells to join the “okazaki fragments” during DNA
It plays an important role in repairing single-strand breaks in duplex DNA in living organisms, but some forms such as DNA ligase IV) may specifically repair
There are many more functions that proteins serve, e g another important role is as an enzyme Like their functions, protein molecules vary tremendously They
Furthermore, the process requires 'biological glue', i e enzymes called ligases, to join the insert and vector together A generic gene cloning process may
ENZYMES IN GENETIC ENGINEERING: RESTRICTION NUCLEASES: EXO The role of DNA ligase is to seal nicks in the backbone of double-stranded DNA
Used in the cloning method • Ex EcoRI from Escherichia coli 5 The function of a restriction endonuclease or enzyme Fig 10 1 Some useful properties of DNA
with the plasmid vector became possible with the enzyme DNA ligase, which acts on cut DNA Each restriction endonuclease functions by 'inspecting' the length of ligase Restriction endonucleases are used in genetic engineering to form