analysis without further optimisation (see “Internal antigens and cell cycle” protocol Materials 1 70 ethanol (in DI water) 2 1x PBS 3 PI solution
facilities-flowcyt-pi_cell_cycle.pdf
The cell cycle profile of a sample can be determined by staining the DNA Propidium Iodide (PI) – Blue/Green lasers, also stains RNA
Flow_TechNotes_Cell-cycle-analysis_20170918.pdf
Cell cycle analysis – Propidium iodide (PI) 1 Harvest approx 1^106 cells and centrifuge at 400 g for 5 min 2 Wash cells once with cold PBS
cell_cycle_analysis_0.pdf
CellCycle Analysis The cell cycle profile of a sample can be determined by staining the DNA with a fluorescent dye and PI cell cycle staining protocol:
cell-cycle-analysis-pi.pdf
Cell Cycle Analysis by Propidium Iodide (PI) Staining Adherent cells: 1 trypsinized 2 suspended in medium + 10 FCS 3 centrifuged (1000 rpm, 5 min)
Cell_Cycle_with_PI.pdf
Commonly used DNA dyes used for flow cytometric cell cycle analysis include propidium iodide (PI), 4',6-Diamidino-2-phenylindole dihydrocloride (DAPI),
Cell%20Cycle%20Tutorial.pdf
Method: 1 Prepare cells appropriately; refer to protocol FC1 'Preparation of cells for flow cytometry' for further information
propidium-iodide-staining-of-cells-for-cell-cycle-analysis.pdf
Cell cycle analysis by quantitation of DNA content was one of the earliest applications of flow In this protocol, PI is used to label DNA content
pi.pdf
*Protocol for staining EtOH fixed cells with PI for cell cycle analysis:* 1 Harvest cells and prepare single cell suspension in buffer (e g PBS
2.pdf