[PDF] arc de précipitation ouchterlony

How does Ouchterlony assay work?

In the Ouchterlony assay, a series of samples (the antigens) are placed in the outer wells of a gel plate, and antibodies (antiserum) are placed in the center well, after which they diffuse out and form different geometric precipitation lines in the gel ( Fig. 1 ).

What causes a precipitin arc?

The antibodies and antigen diffuse through the gel, causing a precipitin arc to form at the zone of equivalence. In this example, only the antiserum in well 1 contains antibodies to the antigen.

What is Ouchterlony double diffusion?

It is more commonly known as Ouchterlony double diffusion or passive double immunodiffusion. In this method, both the antigen and antibody diffuse independently through agar gel in two dimensions, horizontally and vertically. The Ouchterlony double immunodiffusion test may be carried out with one or more of the following objectives:

What is Ouchterlony simulated laboratory experiment?

Thus, this simulated laboratory experiment is an inexpensive, safe, and fast exercise that allows students to observe precipitations reactions of the Ouchterlony assay within the class session time. KEYWORDS: Ouchterlony assay, immunodiffusion, inorganic compounds, simulation, classroom activity, antibody, antigen