[PDF] Prevention of posterior capsule opacification through intracapsular

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1SCIentIfIC RePORTs | (2018) 8:12739

Prevention of posterior capsule

tissue

D'Antin

Barraquer

Tresserra

O O O withbiologicalprocesses(suchasTGF- mechanicallypreventPCOformation 8 11 ,12 ,Mitomycin-C 13

Pirfenidone

14 ,Rapamycin 15 ,Ricin 16 whichseemstobethemoste?ectivesubstance 18 Institut Universitari Barraquer, Universitat Autònoma de Barcelona, Barcelona, Spain.

Centro de Oftalmología

Barraquer, Barcelona, Spain.

Universitat Internacional de Catalunya, Barcelona, Spain.

Department of Pathology,

Institut Universitari Dexeus, Barcelona, Spain. Correspondence and requests for materials should be addressed to

R.I.B. (email: prof.rafael@barraquer.com)Received: 9 April 2018 A ccepted: 14 August 2018 P ublished: xx xx xxxx www.nature.com/scientificreports/

2SCIentIfIC RePORTs | (2018)8:12739

19 2

Od)andhydrogenperoxide

(H 2 O 2 ).H 2

Odsinceithasbeentestedmanytimesbefore

11 ,13,17,20-22 andgivesusapointofreferencewithother 2 O 2 wasselectedduetoitsknownpresencein thelens 23
2 O 2 ,resultincell 24
.LowlevelsofH 2 O 2 havealsobeenshowntopromotefaster woundhealingincorneaboth invitroandinvivo 25
itsuse. water(H 2

Od)and8treatedwithhydrogenperoxide(H

2 O 2 etal. 19 .?ecorneoscleraldiskwasremoved inasinglepiece. needlesthroughtheciliarybody.A?erwardstheiriswasremovedwithforceps(Fig. 1).

Figure 1

A beingplacementinculturemedia.( B oflensepithelialcells. www.nature.com/scientificreports/

3SCIentIfIC RePORTs | (2018)8:12739

mmcontinuouscircularcapsu- thestartingpointofourculturesandtoassurethatwestartedfromaLECsmonolayer.(SupplementaryFig. S1 andTable S1). Treatmentswereperformedusinganirrigationdevicedesignedbyourgroup(Fig. 2). 21
.Ourdeviceconsistsofa16 mmsiliconetube(1mm mmsiliconetube(2mmouter 30
mMH 2 O 2 orH 2

Odduring5

Telstar

minutes.A?erthethirdwash, weretobeculturedforonemonth. withanormal2.5xPlan-objective,givingdark?eldimages(Fig. 3A,CandE).?isallowedfortheevaluationof (Techniquepreviouslydescribedby"Eldred etal."andnamedmodi?eddark?eld) 26
;wetermedthese"tiltillu minationimages"(Fig. 3B,DandF).?istechniquegivesbetterimagesoftheindividualcells,facilitatingthe visualizationofcellularmigrationandmorphologyontheposteriorcapsule(Fig. 4). (Fig. 3C,D)orwrinkles;foldsontheposteriorcapsulecausedbylenscellmigrationandmyo?broblasticdi?eren- tiationresultinginvisualdistortions 3 (Fig. 3E,F). -),low(X),medium(XX)andhigh(XXX)degreeof

eitherwrinklesorcellulardistortions(Fig. 5).Webasedthisscaleonthelowestandhighestdegreesofdistortions

observedinoursamples. con?uencewasreached. imagesasseeninFig. 4.?eseimageswerethenprocessedusingImageJ 27
,givingtheareainpixelswhichwe passedtomm 2

Figure 2

A B )Visualizationoftreatment solutiondistributionfromring.Scalebar =2mm. www.nature.com/scientificreports/

4SCIentIfIC RePORTs | (2018) 8:12739

Figure 3

A C E )Dark?eldand( B D F di?erenttypesandstagesofPCO.( A B duringhydrodissection.( C D E F =400μm.

Figure 4

sampleC031on( A )day1,( B )day8,( C )day10and( D =1mm. www.nature.com/scientificreports/

5SCIentIfIC RePORTs | (2018)8:12739

expressedinmm 2 /day. andeosinformorphologicalanalysis, formationandintercapsularadhesion 28
-30 31
andvimentin(clone 32
.Allimmu nostainingsweredonewiththeBenchMark

α-SMA,

signi?cant. TissuesampleinformationandageneraloverviewcanbeseeninFig. 5.Sampleswereculturedforanaverageof SomeH 2

Odsamplesstartedshowingsigns2weeksinandH

2 O 2 samplesonlystartedshowingsomesignsof inculturedcontrolsthanintreatedsamples(Fig. 5).VisualexamplesofthesedegreescanbeseeninFig. 6,at day30:C034istransparent( )buta distortions.OneculturedcontrolandtwoH 2 couldbeobtainedsotheywerediscarded. increased.Ascellsstarttomigrate;they?rstconcentrateontheequatorofthecapsule(Fig. 6).Sometimesthese exampleofcellulardistortioncanbeseeninFig. 6,H 2

Oddays20and30.?eH

2 O 2 treatmentkepttheposterior capsuletransparentinhalfofthesamples,includingthesampleshowninFig. 6. 5H 2

Odsamplesshowedgrowthonday14and4of8H

2 O 2 day22-32)(Fig. 5). ofH 2

OdandallH

2 O 2quotesdbs_dbs33.pdfusesText_39

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