Technical Guide for Non-Radioactive Nucleic Acid Labeling and
HRP-SA and TrueBlue peroxi- dase substrate: Orcein and. Eosin Y counterstains. Southern and Northern blotting; mRNA in situ hybridization. Detection of single.
HiPer® Southern Blotting Teaching Kit
Southern blotting or Southern hybridization is a widely used technique in peroxidase (HRP)-conjugated streptavidin which attaches to the hybridized DNA.
TECHNICAL RESOURCE - Strip Northern and Southern Blots
Following exposure of a Northern or Southern blot (either by classical 17095) test for stripping efficiency by re-applying streptavidin-HRP and add ...
Untitled
solanacearum hrp genes and hrp gene clusters of P. syringae pv. formed Southern blot hybridizations between the three. P. solanacearum hrp genes ...
Southern Blot Chemiluminescent Detection System For Biotin
AuroraTM Southern Blotting kits are available in 2 sizes and sufficient for either 10 or 30 blots ( 10 cm x 10 cm). The reagents included in each kit are.
Isolation of a Gene Cluster from Xanthomonas campestris pv
pathogenicity are controlled by hrp (hypersensitive reaction. and pathogenicity; Niepold et al. exchange mutants were analyzed in Southern blot.
Cheluminate-HRP PicoDetect
Enhanced ChemiLuminescence Detection Kit for Western / Southern / Northern Blotting. Product-No. A3417. Description. The CheLuminate-HRP PicoDetect is a
FORENSIC BIOCHEMISTRY: SOUTHERN AND WESTERN BLOT
Southern blot is used for transferring. DNA the Northern blot for Southern blotting combines agarose gel ... in Western blots is HRP
Understanding secondary antibodies
HRP. Dot ELISA
Lumigen ECL Plus
blotted proteins. Lumigen ECL Plus utilizes a unique Western Blotting HRP Substrate ... This image from a Southern blot of a single copy gene of mouse.
[PDF] Cheluminate-HRP PicoDetect - ITW Reagents
The CheLuminate-HRP PicoDetect is a complete kit with ready-to-use reagents for chemiluminescent detection of immobilized proteins (Western blot) or
[PDF] KPL Detector™ HRP Chemiluminescent Blotting Kit - SeraCare
The KPL Detector HRP Chemiluminescent Blotting Kit was designed for the detection of plasmid and multiple-copy genomic Southern blots Using KPL LumiGLO
[PDF] SOUTHERN AND WESTERN BLOT TECHNIQUES DNA PROFILING
Southern blot is used for transferring DNA the Northern blot for RNA and the in Western blots is HRP a small stable enzyme with high specificity and
[PDF] Western- & Southern-Blotting Carl Roth
Transfer membrane for protein analyses Southern- and Northern-Blots Made of 100 nitrocellulose HRP- conjugate (rabbit) enables the marker to be
[PDF] Strip Northern And Southern Blots - Thermo Fisher Scientific
Following exposure of a Northern or Southern blot (either by classical isotopic 17097) test for stripping efficiency by reapplying streptavidin-HRP and
Short Technical Reports - Future Science
Schematic diagram of the sequential detection of CFTR genotypes with HRP and AP substrates Page 3 Short Technical Reports Southern Blot Analysis of CFTR
Preparation of horseradish peroxidase-labeled probes - PubMed
The direct labeling of nucleic acid probes with horseradish peroxidase (HRP) may including Southern blots Northern blots colony and plaque screening
[PDF] Western Blotting and ELISA Techniques - ResearchGate
The name Western blotting is a pun on the name Southern blotting a technique for DNA detection and the detection of RNA is termed northern blotting 2 1 Brief
[PDF] Strip Northern and Southern Blots
17095) test for stripping efficiency by re-applying streptavidin-HRP and add substrate as indicated in the protocol If no signal is detected then stripping
Methods Used to Detect Proteins and Nucleic Acids Bound to
HRP-Luminol system: The reaction between HRP and luminol can also be applied for detection of nucleic acids in Southern and Northern blotting
TECHNICAL RESOURCE
In the USA call: 800-8-PIERCE (800-874-3723) or 815-968-0747 • Fax: 815-968-7316 or 800-842-5007 • www.piercenet.com
1Introduction
Following exposure of a Northern or Southern blot (either by classical isotopic methods or North2South
Chemiluminescent
Technology), researchers often wish to strip off the first probe and detecting reagents so that they may reprobe for the same
or different target. This Tech Tip describes several commonly used conditions for stripping probe from a Northern or
Southern blot while maintaining the nucleic acid target on the membrane (Table 1).Be aware that any stripping procedure has the potential to denature sensitive nucleic acids, rendering them unrecognizable by
the probe. Should this occur, reprobing will not be possible, and a new blot will have to be prepared for each probing
experiment. Furthermore, any physical defects of a membrane will be exaggerated when stripped and reprobed.
Table 1. Possible stripping conditions for Northern and Southern blots.Stripping Solution Strength DNA RNA Temperature & Time 0.5% SDS Mild Yes Yes 60°C for 60 minutes
5 mM Tris•HCl, pH 8.0, 2 mM EDTA,
0.1X Denhardt's Reagent Mild Yes Yes 65°C for 2 hours
50% Formamide, 2X SSPE Moderate Yes Yes 65°C for 1 hour
NaOH, 0.1% (w/v) SDS* Moderate Yes No 45°C for 30 minutes, then room temperature for 2 x 10 minutes Boiling 0.1% SDS Harsh Yes Yes Pour on 100°C solution and let cool to room temperature; repeat 2 times0.1X SSPE or SSC/0.5% SDS Harsh Yes Yes Pour on 100°C solution and let cool to
room temperature; repeat 2 times *Blots must be neutralized in TE after stripping.Tips for Stripping and Reprobing Blots
Wash blots with target side up
Handle blots only at corners
Keep blots wet between hybridization and stripping Keep blots wet after stripping
Store blots in 0.1% SDS or 1X TE at 4°C
Stripped blots may be dried and stored at room temperature for long-term storage. However, any probe remaining on the
blot will become permanently bound once the blot is dried.Test for Stripping Efficiency
For biotinylated probes detected using North2South Chemiluminescent Hybridization and Detection Kit (Product No.17095), test for stripping efficiency by re-applying streptavidin-HRP and add substrate as indicated in the protocol. If no
signal is detected, then stripping was complete. If signal is detected, try a stronger stripping method.
For probes directly labeled with HRP and directly detected using North2South®Direct HRP Labeling and Detection Kit
(Product No. 17195), add substrate as indicated in the protocol. If no signal is detected, then stripping was complete. If signal
is detected, try a stronger stripping method.3747 N. Meridian Road
P.O. Box 117
Rockford, IL 61105
TR0028.0 Strip Northern and Southern Blots
In the USA call: 800-8-PIERCE (800-874-3723) or 815-968-0747 • Fax: 815-968-7316 or 800-842-5007 • www.piercenet.com
2Buffer Formulations
50 X Denhardt's Reagent 20 X SSPE
5 gm Ficoll
5 gm Polyvinylpyrrolidone
5 gm BSA
Adjust volume to 500 ml 800 ml H
2 O175.3 gm NaCl
27.6 g NaH
2 PO 4 •H 2 O pH to 7.4 with NaOHAdjust volume to 1 L
20 X SSC TE, pH 7.4
800 ml H2O
175.3 gm NaCl
88.2 g sodium citrate
pH to 7.0 with NaOH Adjust volume to 1 L 10 mM Tris•HCl (pH 7.4)1 mM EDTA (pH 8.0)
Related Pierce Products
77016 Biodyne
B Nylon Membranes, 25/pkg.
17095 North2South
Chemiluminescent Hybridization and Detection Kit, for use in detecting biotinylated probes17195 North2South
Direct HRP Labeling and Detection Kit, for direct labeling of probes longer than 50 nucleotides and subsequent Northern or Southern blotting detection89880 Chemiluminescent Nucleic Acid Detection Module
20148 LightShift
Chemiluminescent EMSA Kit
©Pierce Biotechnology, Inc., 7/2004. Printed in the USA.quotesdbs_dbs14.pdfusesText_20[PDF] hsa singapore
[PDF] hsa software as a medical device
[PDF] hsb course code
[PDF] hsbc bacs calendar 2019
[PDF] hsbc bank transfer time
[PDF] hsbc bsa
[PDF] hsbc corporate banking
[PDF] hsbc currency exchange rate
[PDF] hsbc currency outlook 2020
[PDF] hsbc deferred prosecution agreement 2012
[PDF] hsbc deferred prosecution agreement 2018
[PDF] hsbc exchange rate converter
[PDF] hsbc exchange rate history
[PDF] hsbc exchange rate today