[PDF] qPCR Quantification Protocol Guide - Boston University

How do you calculate serial dilutions?

• Move Volume = Final Volume/(DF -1)
• Diluent Volume = Final Volume – Move Volume.
• Total Mixing Volume = Diluent Volume+Move Volume.
• Example 1: Make a 7-point 1:3 standard curve,starting Neat,such that you can pipette duplicates of 50 μL per well.
• Calculations:

How to calculate serial dilutions?

• Determine the number of dilutions,dilution factor (or range) and starting solution concentration. ...
• Work out how much of the solution you require for each dilution. ...
• Calculate how much of the solution you need to pipette from one dilution to the next. ...
• Make the starting solution. ...

What is dilution method?

• In the laboratory, this method is used to decrease the counts of cells within a culture to simplify the operation. In serial dilution, the cell count or density gradually decreases as the serial number increases in each step.

What is limiting dilution?

• Limiting dilution analysis attempts to determine the frequency of cells having a particular function that are present in a mixed population of cells. So limiting dilution analysis is an easy-operated method that used to measure the abundance of cells able to perform a particular function.

Dilution Protocol When to dilute Dilutions should only be performed when a test value is outside the reportable range or when the sample contains interfering substances (e.g., medications) that cause a nonlinear or invalid result. The Catalyst Dx* analyzer supports automated dilutions (the analyzer mixes the