Identification of a New Vertebrate Nucleoporin, Nup188, with
nucleoporins and interactions between those nucleoporins We have developed a novel two-step “organelle trap” assay involving affinity selection and in vitro pore assembly In the first step, soluble proteins derived from Xenopus egg extracts are applied to a column containing a ligand of interest
Reduced expression of AtNUP62 nucleoporin gene affects auxin
brate Nup62 nucleoporins [6, 14] It is located in the central channel of nuclear pores, together with AtNUP54 and AtNUP58, which are also FG nucleo-porins [6, 15] AtNUP62 is not homologous to other Arabidopsis FG nucleoporins, and it is the only one harbouring the Nsp1-C domain characteristic of yeast and vertebrate Nup62 [6] Arabidopsis
Natively Unfolded Nucleoporins Gate Protein Diffusion across
Natively Unfolded Nucleoporins Gate Protein Diffusion across the Nuclear Pore Complex Samir S Patel,1,2 Brian J Belmont,2 Joshua M Sante,2 and Michael F Rexach1,* 1MCD Biology, University of California Santa Cruz, Santa Cruz, CA 95064, USA
A Putative Arabidopsis Nucleoporin, AtNUP160, Is Critical for
of individual nucleoporins have been extensively studied in yeast and vertebrates, very little is known about NPCs in plants Recently, it was reported that the Arabidopsis thaliana pro-teins MOS3/SAR3 and SAR1 share high sequence similarities with human nucleoporins Nup96 and Nup160, respectively (25, 34)
Flexible phenylalanine-glycine nucleoporins as entropic
nucleoporins (or Nups) that form an eightfold symmetric central framework embracing a central pore The cross-section of the central pore reveals an hourglass-like channel that is 90 nm long and is narrowest (diameter of 40 nm) at the NPC’s midplane (1) Whereas small molecules such as water and ions proceed freely by
Role of Nucleoporins and Transport Receptors in Cell
nucleoporins is very important in transportation of these proteins in various cellular processes, including the cell differentiation This review will cover the role of various Nups and transport
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