Flexible phenylalanine-glycine nucleoporins as entropic

nucleoporins and interactions between those nucleoporins We have developed a novel two-step “organelle trap” assay involving afﬁnity selection and in vitro pore assembly In the ﬁrst step, soluble proteins derived from Xenopus egg extracts are applied to a column containing a ligand of interest

Reduced expression of AtNUP62 nucleoporin gene affects auxin

brate Nup62 nucleoporins [6, 14] It is located in the central channel of nuclear pores, together with AtNUP54 and AtNUP58, which are also FG nucleo-porins [6, 15] AtNUP62 is not homologous to other Arabidopsis FG nucleoporins, and it is the only one harbouring the Nsp1-C domain characteristic of yeast and vertebrate Nup62 [6] Arabidopsis

Natively Unfolded Nucleoporins Gate Protein Diffusion across

Natively Unfolded Nucleoporins Gate Protein Diffusion across the Nuclear Pore Complex Samir S Patel,1,2 Brian J Belmont,2 Joshua M Sante,2 and Michael F Rexach1,* 1MCD Biology, University of California Santa Cruz, Santa Cruz, CA 95064, USA

A Putative Arabidopsis Nucleoporin, AtNUP160, Is Critical for

of individual nucleoporins have been extensively studied in yeast and vertebrates, very little is known about NPCs in plants Recently, it was reported that the Arabidopsis thaliana pro-teins MOS3/SAR3 and SAR1 share high sequence similarities with human nucleoporins Nup96 and Nup160, respectively (25, 34)