HPLC Basics – principles and parameters
interaction HPLC [1] In Fig 3 an analytical and a preparative column are shown Detector The task of the detector unit is to register the time and amount of a substance which is eluted from the column The detector perceives the change in the composition of the eluent and con -
Preparative Chromatography Overview - Flowspek
Analytical and preparative HPLC systems possess in principle a similar design but have completely different system requirements, particularly with regard to the pumping systems The Knauer Smartline 1800 Pump ofers flowrates up to 1000 ml per minute with 4 different pump heads for optimum combination of flow and pressure capability
An Introduction to High Performance Liquid Chromatography
High Performance Liquid Chromatography, or HPLC, is the most common analytical separation tool and is used in many aspects of drug manufacture and research HPLC is used for: 1 Qualitative and quantitative analysis of unknown mixtures – determining what is there, and how much 2 Separation of mixtures for later analysis –preparative HPLC
CHROMATOGRAPHIE LIQUIDE HAUTE PERFORMANCE (HPLC)
haute performance (HPLC) • la chromatographie en couche mince (CCM) Les deux premières méthodes peuvent être assez largement décrites par des théories communes Dans les deux cas, un fluide appelé phase mobile parcourt un tube appelé colonne Cette colonne peut contenir des "granulés" poreux (colonne remplie) ou être
A Review on High Performance Liquid Chromatography (HPLC)
High Performance Liquid Chromatography which is also known as High Pressure Liquid Chromatography It is a popular analytical technique used for the separation, identification and quantification of each constituent of mixture HPLC is an advanced technique of column liquid chromatography The solvent usually flows through
HPLC Troubleshooting Guide - Sigma-Aldrich
Figure A Components of an HPLC System Column and Integrated Guard Column Solvent Return Line Isolating HPLC Problems In an HPLC system, problems can arise from many sources First define the problem, then isolate the source Use Table 1 (page 5) to determine which component(s) may be causing the trouble A process of elimination will usually
Les Bonnes Pratiques en HPLC t UPLCHPLC et UPLC
HPLC t UPLCHPLC et UPLC Les clés de la réussite pour vos analyses en HPLC et/ou UPLC Philippe Vassault Responsable Business Colonne – Europe du Sud i h L CL aura Charrier Ingénieur Support Technique - France ©2015 Waters Corporation 2 Posez vos questions à tout moment en utilisant la boite de dialogue
Chromatographie Liquide à Haute Performance HPLC
hplc La chromatographie liquide à haute performance, souvent appelée du nom de son abréviation CLHP – HPLC en langue anglaise – constitue une technique analytique
TECHNIQUES: Principes de la chromatographie
Les séparation sont basées sur le principe de polarité, c’est-à-dire l’existence de dipôles dans une structure moléculaire Adsorbants possibles (du moins polaire au plus polaire): Papier, cellulose, amidon, carbonate de sodium, gel de silice, alumine, charbon activé 8 Adsorbants Les adsorbants sont sous forme de granules calibrés
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