Maximize yield, purity, and integrity

EURL-GMFF: verification report for extraction of DNA from pollen in honey CTAB-based DNA extraction (eventually followed by a column-purification step in case inhibiting/interfering substances would still be present) 4 Method A detailed description of the extraction method for genomic DNA of pollen in honey samples is given in Annex I

Maize Seeds Sampling and DNA Extraction Report on the

The CRL-GMFF carries out all its operations according to ISO 9001:2000 (certificate number: CH-32232) and ISO 17025:2005 (certificate number: DAC-PL-0459-06-00) [DNA extraction, Extraction - Report on the Validation of a DNA Extraction Method from Maize Seeds”

Report on the In-house Validation of a DNA Extraction Method

EU-RL GMFF: validation report maize DNA extraction JRC publication JRC102145 4/15 1 Introduction This report describes the validation of a ‘CTAB-based’ DNA extraction method (1-3) followed by further purification of the extracted DNA by applying the commercially available Wizard ® mini column DNA clean-up system (Promega)

The method below was developed by Genetic ID NA, Inc as a

DNA Extraction For DNA extraction and purification the silica membrane column based Fast ID Genomic DNA Extraction kit was compared to the Machery&Nagel Plant II kit Subsequent validation was performed using the Fast ID kit only Real-time PCR system The PCR system uses standard cycling conditions with Taqman® chemistry for a singlex PCR system

Definition of Minimum Performance Requirements for Analytical

according to parameters described in Annex 2 For the specific case of DNA extraction modules already validated by the EURL GMFF, no further verification is required, provided that the DNA extraction module has been declared fit for the purpose for the same species and on the same matrix 2 1 Acceptance criteria common to all modules of a method

RESEARCH ARTICLE Open Access Testing the interaction between

including DNA extraction and subsequent quantification by PCR [7] The whole analytical procedure for GMO quantification in food and feed consists of several, add-on sequential steps, from sample preparation to DNA extraction, purification and real-time PCR measurement The result is provided in the form of a ratio between

Final report of CCQM-K86c Relative quantification of genomic

Extraction method (sample intake) Instrument PCR Reagents Primers and probes used for T1 Primers and probes used for T2 JRC Geel (IRMM) CTAB-Tip20 (200 mg) BioRad QX-100 + C1000 Touch™ Thermo cyclerThermal Cycler ddPCR™ Supermix As defined in Appendix A DP-073496-4 fatA(A) As defined in Appendix A RT73 fatA NIMT EURL GMFF protocol (200 mg)

Report on the In -house Validation of a DNA Extraction Method

This DNA extraction method is applicable to a variable amount of starting material, and three extraction scales are described, a small, a medium and a large scale The data provided by the applicant are derived from a large scale DNA extraction, while the EU-RL GMFF performed the DNA extractions with the small scale version of the protocol

Extraction, Purification and Production of Enzymes

1 LARGE-SCALE EXTRACTION AND PURIFICATION OF ENZYMES AND OTHER PROTEINS •Extraction by Chemical Methods •Alkali •Lysozyme and EDTA •Detergents •Cold Shock •Osmotic Shock •Extraction by Physical Methods •Sonication •Freezing and Thawing