AAV has not been identified as the causative agent of human disease.
However, AAV has been associated with several conditions affecting human reproduction.
The presence of AAV in testicular tissue and semen is associated with male infertility..
Generally, Adenovirus is classified as a biosafety level 2 organism.
Adenoviral vectors may be regulated at varying biosafety levels, depending on the nature of the inserted genes and its replication competence.
Experiments using Adenovirus require local IBC approvals before initiation of experiments..
AAV vectors are biosafety level 1, and consist of recombinant transgene sequences (e.g. marker or human genes) flanked by the AAV inverted terminal repeats.
The removal of the majority of viral structural genes renders the vector replication-defective and dependent on an AAV helper virus..
Generally, Adenovirus is classified as a biosafety level 2 organism.
Adenoviral vectors may be regulated at varying biosafety levels, depending on the nature of the inserted genes and its replication competence.
Experiments using Adenovirus require local IBC approvals before initiation of experiments..
Generally, Adenovirus is classified as a biosafety level 2 organism.
Adenoviral vectors may be regulated at varying biosafety levels, depending on the nature of the inserted genes and its replication competence.
Experiments using Adenovirus require local IBC approvals before initiation of experiments..
AAV vectors are biosafety level 1, and consist of recombinant transgene sequences (e.g. marker or human genes) flanked by the AAV inverted terminal repeats.
The removal of the majority of viral structural genes renders the vector replication-defective and dependent on an AAV helper virus.
Do not manipulate AAV in a biological safety cabinet at the same time as Risk Group 2 materials.
Decontaminate work areas before and after use.
If experiments with AAV also include:
Made in the absence of helper virus (such as:
Adeno-associated virus (AAV) and recombinant adeno-associated virus (rAAV) are commonly used for gene expression with fewer associated biosafety concerns when compared to viral vectors that are persistent and able to integrate into the genome.
The following is a brief synopsis of IBC guidance relevant to biosafety with respect to AAV/rAAV vectors.
