Large pools of fetal calf serum may be contaminated with bovine pathogens, when one or a few calves carried infectious agents.
This may cause a biohazard if further processing cannot remove the pathogens, such as bovine viruses or possibly even the causative agent of bovine spongioform encephalopathy..
Furthermore, researchers rely on FBS that is sterile, filtered, and free of mycoplasma.
Thermo Fisher Scientific's commitment to quality and consistency helps ensure that each lot of Gibco sera meets the needs and standards necessary for optimal cell culture performance and growth..
Fetal bovine serum (FBS) is the most widely used growth supplement added to in vitro cell cultures.
It has been in use for over fifty years, outperforming both synthetic and natural growth supplements..
What is fetal bovine serum used for? The primary use of FBS is as a growth supplement for in vitro cell culture, and it is typically added to basal cell culture media at a concentration of 5–10%..
Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells.
This is due to it having a very low level of antibodies and containing more growth factors, allowing for versatility in many different cell culture applications..
Fetal bovine serum used as a supplement in culture media primarily aids in: Supplying hormone factors for cell proliferation and growth.
Providing nutrients, trace elements, transport proteins, adherence, and extension factors.
Cultivating a suitable environment for growth with stabilizing and detoxifying factors..
In cabinet laboratories, all work must be done within a class III biosafety cabinet.
Materials leaving the cabinet must be decontaminated by passing through an autoclave or a tank of disinfectant.
The cabinets themselves are required to have seamless edges to allow for easy cleaning.
