Protein crystallography pdf

  • How to do protein crystallization?

    Protein Crystallization Steps

    1. Choose an appropriate solvent
    2. Achieve supersaturation
    3. Crystallize the product by controlling CPPs
    4. Allow the system to reach equilibrium
    5. Filter and dry the purified product

  • Stage 1 – Protein Production (target crystal selection), Stage 2 – Crystallisation, Stage 3 – Data Collection, Stage 4 – Phasing (data processing), Stage 5 – Solution of digital protein structure model, Stage 6 – Submission of protein model into public database.

What are the parameters of protein crystallization?

The parameters that can be varied include: nature and concentration of the precipitating agent; buffers to explore the entire pH range; additional salts, detergents, and ligands that affect the solubility of the protein; and others

Usually to obtain protein crystals several crystallization conditions should be explored

What is protein crystallography?

Authoritative and practical, Protein Crystallography aims to ensure successful results in the further study of this vital field


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