Serial crystallography microfluidics

  • How does microfluidics work?

    How do microfluidics work? Microfluidics systems work by using a pump and a chip.
    Different types of pumps precisely move liquid inside the chip with a rate of 1 μL/minute to 10,000 μL/minute.
    For comparison, a small water drop is ~10 microliter (μL)..

  • What are the techniques used in microfluidics?

    Microfluidic techniques such as droplet microfluidics, paper microfluidics, and lab-on-a-chip are used in the realm of food science in a variety of categories.
    Research in nutrition, food processing, and food safety benefit from microfluidic technique because experiments can be done with less reagents..

  • What is micro seeding method of protein crystallization?

    Microseeding is a method used in protein crystallography where small seed crystals can be used to induce the formation of larger crystals.
    Microseeding was first shown to generate untwinned crystals of a compound called LigM, an O-demethylase, using untwinned crystals as seeds..

  • What is the mechanism of microfluidic device?

    Microfluidic devices contain a palm-sized chip imprinted with very small channels and reaction chambers used to detect antibodies against viruses.
    The chip uses electrokinetic, capillary, vacuum, and other forces to mix and separate liquid samples..

  • Microfluidics refers to a system that manipulates a small amount of fluids (10−9 to 10−18 liters) using small channels with sizes ten to hundreds micrometres.
    It is a multidisciplinary field that involves molecular analysis, molecular biology, and microelectronics.
  • Microseeding is a method used in protein crystallography where small seed crystals can be used to induce the formation of larger crystals.
    Microseeding was first shown to generate untwinned crystals of a compound called LigM, an O-demethylase, using untwinned crystals as seeds.
Apr 7, 2017The advent of serial crystallography has opened up the potential for time-resolved experiments to smaller crystals and targets that are highly  INTRODUCTION AND Droplet microfluidicsIV. PLATFORMS FOR SERIAL
May 24, 2016This architecture allows for a total material thickness of only ∼1 μm, facilitating on-chip X-ray diffraction analysis while creating a sample 

Can 3D microfluidic devices be printed for serial crystallography?

There is a need for more robust sample delivery methods for serial crystallography

Here the authors present the design and characterization of ultracompact 3D microfluidic devices that can be printed, which require less sample, have a lower background signal and allow 3D mixing for time resolved experiments

What is Serial femtosecond crystallography (SFX) at X-ray free-electron lasers?

Serial femtosecond crystallography (SFX) at X-ray free-electron lasers seeks to extend the sensitivity and applicability of X-ray diffraction to structural biology

It has allowed the observation of radiation-sensitive structures at the atomic scale 5, 6, 7


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