How do you use cytology fixative spray?
Directions for use:
1 – Prepare smear on the slide by the method normally employed by the laboratory. 2 – Apply sufficient fixative to wet the entire smear.
Spray should be operated about 3-4 inches ( 75-100 mm) from the slide. 3 – Place slide flat in clean , warm area to dry ( 10-15 minutes)..
What is a cytological fixative?
Cytology Fixative covers cells with a tough, soluble film that protects cell morphology for microscopic examination.
It is water and alcohol soluble, environmentally friendly and extremely economical..
What is a fixative and spray in Pap smear?
Fixation spray for cytology is a special fixative in spray form designed to preserve cellular specimens, especially smear specimens such as those used in a Pap smear.
Fixation is an important step in preserving cellular structure and morphology during specimen processing, staining and analysis..
What is the best fixative for cytology?
Ethyl alcohol (95%) is the most commonly used fixative in cytology.
The cytology samples are processed commonly by direct smear, centrifugation, cytocentrifugation, liquid-based preparation, Millipore technique and cell block..
What is the fixative method in cytology?
Ethyl alcohol (95%) is the most commonly used fixative in cytology.
The cytology samples are processed commonly by direct smear, centrifugation, cytocentrifugation, liquid-based preparation, Millipore technique and cell block..
What is the spray fixative used for Pap smears?
Various fixatives are used in exfoliative cytology.
Out of which, 95% ethanol is the commonly used fixative.
Hence, the conventional method for fixation of Pap smear is to fix the Pap smear immediately in 95% ethyl alcohol after preparing the smear..
Which fixative is best used for cytology?
The most commonly used fixatives for diagnostic pathology and cytologic specimens are 10% NBF and 95% ethanol [3]..
- CYTOFIX\xae is an aerosol preparation used to fix biological smears applied on the microscopic slide, prior to their subsequent evaluation.
- Fixation involves the linking together of molecules of the fixed tissue compounds and is, therefore, always accompanied by morphological and chemical change.
A good morphological fixation is one in which the changes occurring in the structures studied occur beyond the resolving power of the optics used. - Fixation spray often contains a mixture of alcohols, acetone and other fixatives that quickly penetrate cell preparations and denature proteins to stabilize cell structures.
This preserves cell morphology and allows for optimal staining.
