Place a clean glass spreader slide in front of the drop of fluid/urine at approximately a 30\xb0–40\xb0 angle to the smear slide. 3.
Back the spreader slide into the drop, allowing the material to spread along the edge of the spreader slide..
Insert the cytobrush into the cervical os no further than the end of the bristles and rotate 90\xb0.
For SurePath: Snap off the head of the cytobrush (avoid touching the head of the device), and drop into the liquid vial (avoid splashing).
For ThinPrep: Rinse the cytobrush by swirling it vigorously in the vial 10 times..
In this, a sample is vortexed and strained to break the mucus and large cell groups and then is treated through a density gradient centrifugation process to remove blood and debris.
The cell pellet is resuspended and is allowed to sediment onto a glass slide.
This is followed by staining on the PrepStain instrument.Jun 14, 2022.
An artificial neural network or another expert system may help in the automated interpretation of the slides
The liquid-based cytology (LBC) is also part of automation that can process the fluid specimen
Presently two widely used techniques of LBC are available in the market: Thin Prep and SurePath
In liquid-based cytology, the brush head of the device is immersed in a vial of fixative solution where it is vigorously rotated several times to ensure release of the cells collected (Fig
26 4 )The device is then removed from the vial
Cytopathology: In cytology, different liquid-based cytology preparation systems are available (ThinPrep and SurePath systems)
These systems are described in the next section of the chapter
Microtomy: In histopathology, the semiautomated microtomes are available
