Which eukaryotic polymerase requires a specific set of transcription factors?
Each eukaryotic polymerase also requires a distinct set of transcription factors to bring it to the DNA template. RNA polymerase I is located in the nucleolus, a specialized nuclear substructure in which ribosomal RNA (rRNA) is transcribed, processed, and assembled into ribosomes.
Which enzyme removes RNA primers?
RNA primers need to be replaced with DNA, and nicks in the sugar-phosphate backbone need to be connected. The group of cellular enzymes that remove RNA primers include the proteins FEN1 (flap endonulcease 1) and RNase H.
What dnaps do eukaryotes use for replication?
Eukaryotes utilise various multimeric DNAPs for replication of the genome; namely Pol a, Pol d and Pol e (Burgers, 2009). All contain a catalytic core identifiable as a Family B polymerase, along with various accessory subunits fundamental to their roles in replication (Hubscher et al., 2002).
What are the types of DNA polymerases?
Based on the sequence similarity and enzymatic properties, DNA polymerases are classified into six main groups: families A, B, C, D, X, and Y . Families B and C are eukaryotic and bacterial replicative DNA polymerases, respectively [2, 3].
Quelles sont les ADN polymérases requises pour la réplication et la