A classical KO mouse line with a deleted Mtm1 allele (MTM1 4 line) was obtained by crossing Mtm1 ‘‘floxed’’ mice (MTML3) with a transgenic line carrying the Cre transgene under a cytomegalovirus promoter (CMV-Cre line), followed by selection of mice with exon 4 germline excision (Fig 1 a–c) This deletion
(F) Percentage of tubules per Z-line in myofibers of 2-week-old mice (P 0 001) Data were obtained from n 16 TA muscle fibers of four WT and four KO mice at both 5 and 2 weeks of age Downloaded at Microsoft Corporation on March 9, 2020 18764 www pnas orgcgidoi10 1073pnas 0900705106 Al-Qusairi et al
specific mutant line (Mtm1/HSA ¼ mKO) to avoid an immunological reaction against the transgene The tibialis anterior (TA) muscle of 4 week-old muscle-specific knockout (mKO) mice was selected for rAAV vector injection, a widely used muscle for gene therapy experiments, because weakness was already present in hindlimbs at this age (animals
cell line enhances both ATP production and insulin secretion PX domain: phox homology domain This domain is named for its presence in the NADPH oxidase p40phox and p47phox subunits It is a phosphoinositide-binding module that is composed of 120 amino acids that are found in diverse eukaryotic proteins
After Chap ter 1 “HTML Primer,” all HTML is sim ply shown to pro vide con text for the JavaScript, and an expla na tion for the HTML code is not usu ally given How ever, only stan dard HTML tech niques are used and the code is pre sented in a very orga nized man ner so that any one with a basic knowl edge of HTML can eas ily fol low along
A survey of Penicillium brevicompactum and P bialowiezense from indoor environments, with commentary on the taxonomy of the P brevicompactum group1 James A Scott, Bess Wong, Richard C Summerbell, and Wendy A Untereiner
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irradiated at 30 J/m2 (Figure 1C), in line with the fact that these cells went into an apoptotic process as they left their substrates (see above) Thus, the minimal dose (10 J/m2) of UVC that induces formation of granules in a high number of cells correlates with cell cycle arrest Intriguing enough, while the number of SGs decreases steadily
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