The cell cycle profile of a sample can be determined by staining the DNA Propidium Iodide (PI) – Blue/Green lasers, also stains RNA
Flow_TechNotes_Cell-cycle-analysis_20170918.pdf
This is a method for cell cycle analysis using propidium iodide (PI), that is, using the fluorescent nucleic acid dye PI to identify the proportion of cells
facilities-flowcyt-pi_cell_cycle.pdf
Cell Cycle Analysis by Propidium Iodide (PI) Staining Adherent cells: 1 trypsinized 2 suspended in medium + 10 FCS 3 centrifuged (1000 rpm, 5 min)
Cell_Cycle_with_PI.pdf
Cell cycle analysis – Propidium iodide (PI) 1 Harvest approx 1^106 cells and centrifuge at 400 g for 5 min 2 Wash cells once with cold PBS
cell_cycle_analysis_0.pdf
Method: 1 Prepare cells appropriately; refer to protocol FC1 'Preparation of cells for flow cytometry' for further information
propidium-iodide-staining-of-cells-for-cell-cycle-analysis.pdf
15 jan 2021 · ab139418 Propidium Iodide Flow Cytometry Kit for Cell Cycle Analysis To determine cell cycle status in tissue culture cell lines by
Propidium-Iodide-Flow-Cytometry-Kit-protocol-book-v3-ab139418%20(website).pdf
7 mai 2018 · Propidium iodide staining of DNA is the classic means of cell cycle analysis The staining procedure takes less than 1 hour of total processing
ab139418_Propidium%20Iodide%20Flow%20Cytometry%20Kit_Lot%20M7814_20180507%20CES%20v2a%20(website).pdf
Some DNA dyes do not stain live cells The sample must be fixed and permeabilized to allow the dye to enter the cells ? Propidium Iodide (PI) – Blue/Green
cell-cycle-analysis-pi.pdf
Cell cycle analysis by quantitation of DNA content was one of the earliest applications of flow cytometry The DNA of mammalian, yeast, plant or bacterial
pi.pdf