Rotaviruses biosafety level

What are the laboratory techniques used to diagnose rotavirus?
Conduct nucleic acid detection PCR assays (for rotavirus alone or in multipathogen panels) or antigen-detection immunoassays on stool specimens to rapidly detect rotavirus RNA or antigens and make a diagnosis..
What biosafety level is rotavirus?
Rotavirus is a Biosafety Level 2 (BSL-2) microorganism and must be used within a BSL-2 facility in a biosafety cabinet (BSC)..
What category is the rotavirus in?
Rotavirus is a genus in the family Reoviridae.
Rotaviruses have three important antigenic specificities: group, subgroup, and serotype.
Group A rotaviruses are major pathogens in humans and animals.
Ten serotypes of human group A rotaviruses are defined by neutralization of one (VP7) of the two outer capsid proteins..
What cellular level is the rotavirus in?
Rotaviruses infect and replicate within the mature epithelial cells at the apex of the villus, and viral progeny exit the cell from the lumenal membrane by a process of transcytosis (2)..
What is the classification of rotavirus?
Rotavirus is a genus in the family Reoviridae.
Rotaviruses have three important antigenic specificities: group, subgroup, and serotype.
Group A rotaviruses are major pathogens in humans and animals.
Ten serotypes of human group A rotaviruses are defined by neutralization of one (VP7) of the two outer capsid proteins..
What is the classification of the rotavirus?
Rotavirus is one of six genera in the subfamily Sedoreovirinae in the family Reoviridae (ICTV, 2011).
Rotaviruses are classified based on the inner shell antigen (VP6), termed the group antigen.
Based on the VP6 antigen, there are seven groups of rotaviruses, designated groups A–G..
What is the mechanism of the rotavirus?
Thus, rotavirus-induced diarrhoea is considered non-inflammatory and has two proposed mechanisms: osmotic diarrhoea due to malabsorption (secondary to enterocyte damage or death, or to decreased epithelial absorptive function) and secretory diarrhoea due to the effects of NSP4 and activation of the enteric nervous .
What is the pathogen of the rotavirus?
What pathogens are responsible for this disease? Rotavirus—the cause of rotaviral gastroenteritis—is a non-enveloped, double-stranded, segmented RNA virus of the Reoviridae family..
What is the serotype of the rotavirus?
Group A rotaviruses are serotyped on the basis of antibody reactivity to either VP6 or VP7, both of which are proteins on the outer shell of the virion (Hoshino and Kapikian, 1996).
The 11-segmented rotavirus genome, in addition to coding for the structural proteins, encodes six non-structural proteins..
Where is rotavirus commonly found?
The reservoir of rotavirus is the gastrointestinal tract and stool of infected humans.
Although rotavirus infection occurs in many nonhuman mammals, transmission of animal rotaviruses to humans is believed to be uncommon and probably does not lead to clinical illness..
Children are most at risk for getting rotavirus disease.
The most severe rotavirus disease occurs primarily among unvaccinated children aged 3 months to 3 years old.
Some adults also have a higher risk of getting rotavirus including those who: are older, care for children with rotavirus disease, or.
Overview.
Rotavirus is a very contagious virus that causes diarrhea.
Before the development of a vaccine, most children had been infected with the virus at least once by age 5.
Although rotavirus infections are unpleasant, you can usually treat this infection at home with extra fluids to prevent dehydration.
Rotavirus is a double-stranded RNA virus of the family Reoviridae.
The virus is composed of three concentric shells that enclose 11 gene segments.
The outermost shell contains two important proteins: VP7, or G-protein, and VP4, or P-protein.
Rotavirus is the leading cause of acute gastroenteritis in the world.
Studies estimate that approximately 200,000 people die annually from infection.
Although the infection rate has decreased with the advent of vaccines, infections are still common throughout the world.
Rotavirus may be identified by several means (ie, enzyme immunoassay being the most common, latex agglutination, electron microscopy, culture).
However, in most cases, identification of the virus is important for public health or infection control purposes.

BIOSAFETY: Rotavirus is a Biosafety Level 2 (BSL-2) microorganism and must be used within a BSL-2 facility in a biosafety cabinet (BSC). Please consult your

Production steps and quality control operations involving manipulations of live rotavirus should be conducted under Biosafety Level 2 (14).

Rotavirus is a Biosafety Level 2 (BSL-2) microorganism and must be used within a BSL-2 facility in a biosafety cabinet (BSC).

Section I - Infectious Agent

NAME: Human rotavirus SYNONYM OR CROSS REFERENCE: HRV, Human reovirus-like agent, infantile gastroenteritis virusFootnote 1, sporadic viral gastroenteritis, severe viral gastroenteritis of infants and children, non-bacterial gastroenteritis of infancy, and rotaviral enteritis.
CHARACTERISTICS: Human rotavirus is classified as a member of genus Rota.

Section III - Dissemination

RESERVOIR: Humans are the only reservoir for HRV; however, infection by group A rotaviruses (GARVs) has been reported in calves, pigs, foals, cats, dogs, and some birds.
The GARVs found in animals appear to be very closely related to HRVFootnote.
8) Evidence for some interspecies infection by GARVs does exist.
More importantly, it has been suggested.

Section IV - Stability and Viability

DRUG SUSCEPTIBILITY: Unknown.
SUSCEPTIBILITY TO DISINFECTANTS: HRV, either in suspension or on inanimate surfaces, is susceptible to glutaraldehyde (2%); chlorinated disinfectants (>20,000 p.p.m. chlorine); iodinated disinfectants (>10,000 p.p.m. iodine); combinations of quaternary ammonium compounds with alcohols (>40%), some acids (HCl), some bas.

Section Vi - Laboratory Hazards

LABORATORY-ACQUIRED INFECTIONS: No cases of laboratory-acquired infection have been reported to date.
SOURCES/SPECIMENS: The main sources of HRV are intestinal mucosa and stool extracts of infected humansFootnote.
8) It has also been detected in rectal swabs of infected humansFootnote.
7) PRIMARY HAZARDS: Ingestion of feces or stool samples and other.

Section VII - Exposure Controls / Personal Protection

RISK GROUP CLASSIFICATION: Risk Group 2Footnote 17.
CONTAINMENT REQUIREMENTS: Containment Level 2 facilities, equipment, and operational practices for work involving infectious or potentially infectious materials, animals, or cultures.
PROTECTIVE CLOTHING: Lab coat.
Gloves when direct skin contact with infected materials or animals is unavoidable. .

Section VIII - Handling and Storage

SPILLS: Allow aerosols to settle.
While wearing protective clothing, gently cover the spill with absorbent paper towel and apply appropriate disinfectant, starting at perimeter and working towards the centre.
Allow sufficient contact time before clean upFootnote 18.
DISPOSAL: Decontaminate, either by steam sterilization, incineration, or chemical d.

Rotaviruses biosafety level

What are the laboratory techniques used to diagnose rotavirus?

What biosafety level is rotavirus?

What category is the rotavirus in?

What cellular level is the rotavirus in?

What is the classification of rotavirus?

What is the classification of the rotavirus?

What is the mechanism of the rotavirus?

What is the pathogen of the rotavirus?

What is the serotype of the rotavirus?