Cytology on csf

  • How is cell count done in CSF?

    For a CSF differential cell count, a lab technician examines the types of WBCs found in the CSF sample and counts them.
    He or she also looks for foreign or abnormal cells.
    Dyes are used to help separate and identify cells..

  • What cells are seen on CSF scan?

    The nucleated cells seen in normal adult CSF are predominantly lymphocytes and monocyte/macrophages.
    A rare neutrophil may be seen.
    An increased number of lymphocytes, monocytes, or neutrophils in CSF is termed pleocytosis.
    Morphologically normal cells can be seen in abnormal numbers in meningitis and inflammation..

  • What investigation is done on CSF?

    CSF analysis usually consists of opening pressure measurement, biochemical analysis, cytology, biomarkers assay, and microbiological evaluation.
    In some clinical conditions, lumbar puncture and drainage of can be a therapeutic measure also..

  • What is the confirmatory test for CSF?

    In patients with chronic, unresolved symptoms suggestive of intracranial hypotension without another overt cause, magnetic resonance imaging (MRI) or digital subtraction myelography can be used to confirm the existence and location of a spinal CSF leak..

  • What tests are done on CSF fluid?

    All of the following can be, but are not always, measured in a sample of CSF:

    Antibodies and DNA of common viruses.Bacteria (including that which causes syphilis, using a VDRL test)Cell count.Chloride.Cryptococcal antigen.Glucose.Glutamine.Lactate dehydrogenase..

  • CSF contains more sodium (Na+) and chlorine (Cl-) than blood, but less potassium, (K+), calcium (Ca++), proteins, and glucose.
    Normal CSF glucose is usually 2/3 of blood glucose.
    Normal CSF protein is 15-60 mg/100 ml and contains no red blood cells (RBC) or clotting factors and only 0-3 white blood cells (or WBC).
Cytological analysis is a critical component of CSF evaluation because the differential count (percentages) of cells may be abnormal, even if the total nucleated count is within reference interval. Cytology also enables examination for neoplastic cells, infectious agents, and evidence of prior hemorrhage.

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