[PDF] Active surveillance of bat rabies in France: a 5-year study (2004-2009)

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Accepted Manuscript

Title: Active surveillance of bat rabies in France: a 5-year study (2004-2009) Authors: Evelyne Picard-Meyer, Marie-Jo Dubourg-Savage,

Laurent Arthur, Michel Barataud, David B

´ecu, Sandrine

Bracco, Christophe Borel, G

´erald Larcher, Benjamin

Meme-Lafond, Marie Moinet, Emmanuelle Robardet, Marine

Wasniewski, Florence Cliquet

PII: S0378-1135(11)00208-2

DOI: doi:10.1016/j.vetmic.2011.03.034

Reference: VETMIC 5257

To appear in:VETMIC

Received date: 31-7-2010

Revised date: 17-3-2011

Accepted date: 31-3-2011

Please cite this article as: Picard-Meyer, E., Dubourg-Savage, M.-J., Arthur, L.,

Barataud, M., B

´ecu, D., Bracco, S., Borel, C., Larcher, G., Meme-Lafond, B., Moinet, M., Robardet, E., Wasniewski, M., Cliquet, F., Active surveillance of bat rabies in France: a 5-year study (2004-2009),Veterinary Microbiology(2010), doi:10.1016/j.vetmic.2011.03.034 This is a PDF le of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. before it is published in its nal form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Active1

2

E1, Marie2,2, 2, 3

Bécu2, 2, 2, Gérald Larcher2, 2, 4

Moi1, 1, Marine Wasniewski1, 1 5

6 1.7 H8 Tele9 11

Corresponding author:12

13 14

Active15

reported in Europe 16 , suggesting previous contact with 17 virus 18 particularly 19 genomes 20 neutrali 21
22

Key words, 23

24

Ab 25

r 26

PCR: 50: 27

c28 29
30

Rabies31

very old32 (World Health Organisation, 2008) ,33

Rhabdoviridae34

bite from35

The 36

2010)³classical´ 37

Mokola virus (MOKV)38

b39 viruses40 the Internatio41 recognised 42 bats 43

Since 44

by EBLV45 (genotype 6) 46 been reported in European countries having a well 47 (G48 to 49 reported 50 ) with 20 cases, of which 51 (Banyard et al., 2009; Harris et asibling 52 species ofand mainly found in Southern Iberia53

EBLV 54

, , , 55

2007) 56

N57

Net Working Group, 2005)58

and epidemiological surveillance59

Here60

with 61 was to investigate the circulation of 62 involved in the63 bats 64

EBLV65

66
67
68

Sampling69

From 70

the country71 to confirmed cases of EBLV 72

Ardennes73

along 74

Bat specialists identified b75

Helve76

bat:77 Oral swabs were taken to collect saliva from each trapped bat and stored i78 (Ambion, France)79 reverse80 culture infection test ( 82 fluorescent antibody virus neutralisation83 released at the 84 a 85 number of 86 87

Laboratory methods88

Oral swabs89

neuroblastoma cells (ATCC:CCL31) 90

2004)91

by 92 primers93 using94 To 95 performed 96

France 97

dilutio98 dog 99 are 50. 100

Spearman50.101

The 102

source 103 104

Presence of EBLV105

From 106

mFAVNt, 107

N50)108

antibody 109 110
111
in 112 with EBLV113 central France114

France115

Of t116

population, seroprevalence among females (n=13; 18.6% seroprevalence; 95% CI, 117 was significantly higher (F2=6.13; P=0.013) 118

CI, 119

120

Presence of infectious 121

All RTCIT samples (n=173) were negative122

saliva samples 123

RNA. 124

positive125 126
127
The purpose of the present study was to investigate the circulation of EBLV128 bat species in France. 129

France , , , 130

).131

Several, 132

, (Serra133 ha 134

Vazquezspecies135

(Klein et al., 2007). 136

Moron et al., 2008b)137

In contrast 138

serologically 139

Belgium 141

Europe to determine the seroprevalence in bats are based 142 the antigen143 bat studies ( 144 differences in levels of 145 the146 bats 147

2003)148

reactivity 149 serologically Lyssavirus), even if the serological test has been adapted to EBLV150

date and despite the reinforcement of bat rabies passive surveillance, no other rabies virus than 151

EBLV152

153

EBLV154

[and 155 seroprevalence 156 presence of antibodies in females suggest157 colonies158 male 159 (Vos et al., 2007)160 rabies ser161 study, males ) were shown to be 162 serologically positive. 163 limited sample164 that 165 radius of about 25 km 166 summer and winter ,167 distances are greater than 350 km 168

France 169

to be170 central Europe 171 roosts 172 173

The effect of 174

of rabies antibodies in bat 175 positive 176 whether the 177 immune response of the host178 exposed to 179 response 180

Spain, indicat181

2007)182

have been 183 phase or ensuing illness184 i 185 contact among bats, through biting, scratching or grooming.186 T187 among bats188

Serra189

share their roosts190 c , 191 offering many 192

2007) 193

194

In the present stu³healthy´ 195

clinical signs of rabies. 196 the study 197 sampling198 et al., 2009b; Johnson et al., 2008)199

2009; Kuzmin et al., 2008)200

oral201 to temporal fluctuations in 202

PCR203

The results of the present study suggest that serologically positive 204 south205 first case of EBLV206 not 207 undertaken208 209
210

Continuing a211

b distribution 212 techniques used in European laboratories working on rabies, there is a 213 standardi214 215
216

M 218

surveillance of bat rabies219

We 220

(221

Servat, 222

Biarnais and Sébastien Kempff 223

Jacques Barrat.224

of the French Ministry of Agriculture226 227
228

2, e566.

154, 1847

11, 207

239

11, 572

155, 133

212, 79

83, 3

39, 3678

Franka, R., Johnson, N., Muller, T., Vos, A., Neubert, L., Freuling, C., Rupprecht, C.E., Fooks,

89, 1998

122, 425

90, 2493

45, 1030

11, 222

89, 2662

14, 1887

29, 5

152, 273

31, 372

22, 1921

13, 1371

3

8, 413

Serra

Miniopterus schreibersii

43, 271

40, 403

125,

Tadarida 321

brasiliensi) at natural and man 10, 165

145, 1919

131,

14, 1263

9, 283

N°99.

408, 183

Figure 1349

given in Table 1) and circle size 350

Figure 1B.351

the 352 during active surveillance353

PCR technique354

355

Table 1:1

2

Region

North-eastern France

Central

France

North-

wester n Franc e

South-westernf France

Species\Site no.

1 2 3 4 5 6 7 9 P 8 10 11 P 12 P 13 P 14 P 15 16 17 P 18 P 3 (0) 1 (0) 1 (0) 3 (3) 1 (0) 2 (1) 2 (2) 2 (2) 9 (2) 2 (2) 7 (0) 3 (0) 1 (1) 2 (0) 1 (1) 1 (1) 3 (0) 11 (0) 33
(0) 12 (12) 2 (2) 7 (7) 1 (1) 1 (1) 5 (5) 2 (0) 2 (0) 1(0) 3 (3) 1 (0) 3 (3) 2 (2) 1 (0) 5 (5) 2 (2) 1 (0) 2 (2) 7 (3) 8 (5) 1 (1) 8 (0) 18 (16) 1 (0) 1 (1) 8 (8) 1 (1) 17 (17) 4 (4) 9 (8)quotesdbs_dbs25.pdfusesText_31

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