Fluid cytology plus cell block

  • How cell block is prepared from different body fluids for the study of cells?

    Preparation of cell block in collodion bag.
    Pipette specimen into collodion bag.
    Centrifuge an aliquot of fluid in the tube containing the collodion bag for 10 minutes at 2,500 rpm.
    Carefully pipette off supernatant..

  • What does cytology and cell block mean?

    Objective: The cell block (CB) technique refers to the processing of sediments, blood clots, or grossly visible tissue fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology..

  • What is a cell block technique in fluid cytology?

    Cell blocks are preparations in which cytologic material is collected and processed as a paraffin embedded block in a manner that is comparable to formalin-fixed paraffin-embedded tissue in surgical pathology..

  • What is the utility of cell block in cytology?

    The use of cell block (CB) preparation is underused in urine cytology (UC) and varies among hospitals.
    In addition to confirming a diagnosis, CBs can be useful in cases of metastatic disease, diagnoses requiring immunohistochemical (IHC) staining, and for ancillary studies..

  • The cell block is prepared from the pellet of centrifuged cell suspension by adding plasma and thrombin to enmesh the cellular material in a clot (Fig. 1).
    Add up to 20 mL of specimen to a falcon tube and centrifuge for 10 minutes at 1,650 rpm.
    After centrifugation, the supernatant is decanted.
  • The cell block technique employs the retrieval of small tissue fragments from a FNA specimen which are processed to form a paraffin block.
  • The cell block technique employs the retrieval of small tissue fragments from a FNA specimen which are processed to form a paraffin block.
    It is widely accepted that cell block technique increases the cellular yield and improves diagnostic accuracy.
Nov 8, 2022The cell block was prepared by centrifuging the specimen of fluid at 2500 rpm for 15 min. The supernatant fluid was discarded and a cell button  AbstractINTRODUCTIONRESULTSDISCUSSIONMissing: plus | Show results with:plus
Cytoblocks prepared from residual tissue fluids and fine-needle aspirations can be useful adjuncts to smears for establishing a more definitive 
Cytological smear and cell block are commonly used to diagnose pleural fluid effusion. However, there is a paucity of information in the literature where a comparison between a cytological smear and a cell block with corresponding pleural biopsy has been done.

Do cytology slides contain blood clots?

Most of the fluids received in the cytology laboratory contain blood clots or small bits of tissue from the lesion.
While preparing the slide, they remain in the bottle and are not available for microscopy.
Cell blocks are also particularly useful when samples are heavily admixed with blood.

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Is pleural fluid cytology positive?

In study cases pleural fluid cytology was positive in 42% cases (84/200) and pleural fluid cell block was positive in 96% cases (192/200) in detecting malignant pleural effusion (p0.06).

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What is body fluid cytology?

Keywords:

  1. Cell block technique
  2. Conventional smear
  3. body fluids
  4. malignant effusions Body fluid cytology is a diagnostic procedure worldwide and its history can be traced back to the 19th century

Lucke and Klebs were believed to be the first investigators who recognized the presence of malignant cells in an ascitic fluid in 1867 [ 1 ].
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What is cell block cytology?

It has diagnostic, therapeutic and prognostic significance [ 2 ].
Cytological examination of body fluids is commonly done by conventional smear (CS) method.
However, the cell block (CB) method is one of the oldest but less commonly used techniques in the evaluation of body fluids even today.


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